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Version 1.0.1 (Updated Apr 22, 2022)

The Guardian Frogs of Borneo

Why Exceptions Matter in Biology and Everyday Life

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1. Overview

Target Subject:

Science

Grades:

5-8

Estimated Time:

150 min. (~3 classes)

Subject breakdown by standard alignments:

Driving Question(s):

  • Across animal species, is there truth to the stereotype that males are “competitive,” while females are “caring”, or is this an oversimplification?
  • Are the smooth guardian frogs of Borneo the first known species of frog with “competitive females” and “caring males”?

Essential Question(s):

  • How can we use scientific methods to identify a general pattern?
  • Why do exceptions matter in science and in life?

Learning Objective(s):

  1. Explain how animal behavior facilitates in survival and reproduction.
  2. Collect, reorganize, and analyze data to identify a pattern (that males tend to have adaptations to compete for mates, while females provide more parental care), and interpret exceptions to that pattern.
  3. Understand that biological data are variable.
  4. Learn to test hypotheses by interpreting authentic scientific graphs and data.
  5. Cite evidence to support explanatory claims.
  6. Integrate multiple lines of evidence to explain why biological exceptions matter to science and society.

Hooks

  • Part 1: Highly interactive card-based activity that simulates data collection + amazing animal videos
  • Part 2: Atmospheric videos of Borneo, first person narrative of an early career Latina scientist + use of authentic data
Keywords:
sex differencesfrogsmating systemsanimal behaviorparental caresexual selection

Lesson Description

This lesson puts students in the role of scientists, collecting and analyzing real data to answer questions. In Part 1, students "collect" data through a card-based activity called "Role With It." Briefly, students get a male or female card of 1 out of 12 species. When their card is called, students introduce it with a "song" and dance that match the attributes of the species it represents. The class then records data on sexual signal exaggeration and parental investment for that species.

Once they have collected all 24 specimens (cards) students analyze this data (for Part 2) to generate a general observation: "across nonhuman animal species, females tend to be more caring (contributing more parental investment), while males tend to be more adapted to attracting and competing for mates." These are known as conventional sex roles. Students then identify sex-role-reversed species, such as jacanas (a bird) and guardian frogs, which have "caring males" and "competitive females".

Part 3 lets students use real data to test whether the Smooth Guardian Frog (Limnonectes palavenensis) is truly the first known sex-role-reversed frog species, with "caring males" and "competitive females". The lesson begins with an atmospheric video that immerses students in the sights, sounds, and mysteries of the island of Borneo. Students are then given a brief introduction to the geography of the region and learn that Borneo is claimed by three Asian countries (Malaysia, Indonesia and Brunei). The rest of the lesson focuses on Dr. Johana Goyes Vallejos' research in the forests of Brunei. Students test hypotheses by synthesizing information and analyzing a scatter plot to determine that this fascinating species is indeed the first frog known to have "caring males" and "competitive females."

The lesson offers a wide variety of opportunities for student movement, collaboration, creativity, and active engagement.

2. Lesson Preview

"Teach it in 15" Quick Prep
  1. Check out lesson multimedia above
  2. Look over Teaching Materials
  3. Read our easy Procedure
  4. Teach it and let us know what you think!

3. Teaching Materials

Classroom/In-person Teaching Materials

Designed to be led by a non-specialist teacher, in person.

*You will need to be logged into a free Google account and click "Use Template" to add files to your Google Drive.

Resources needed:
1 Digital Items
Presentation x 2 (for Parts 1&2 and Part 3)
3 Printed Items
Teacher Worksheet x 2 (for Parts 1&2 and Part 3)
Student Worksheet x 2 (for Parts 1&2 and Part 3)
"Role With It Cards" (Class set for Part 1: Print enough so each student has at least 1)
5 Other Requirements
Teacher's computer
Internet Connection
Projector
Speakers
Scissors (for cutting out cards)
Grades 5-8

Students "collect" data in a fun activity blending creative movement, cards, animal videos, and data literacy. Don't be intimidated by the length of the presentation–you will zip through the slides! In Part 2, students will analyze this data.

  1. Presentation

  2. Student Worksheet for Parts 1 & 2

  3. Teacher Worksheet for Parts 1 & 2

  4. "Role with It" Activity Cards (Print enough so each student has at least 1)

Students organize, analyze, and interpret the data they collected in Part 1. They will define the pattern of conventional sex roles from the data (most species have "caring females" and "competitive males"), and identify exceptional species that buck the trend.

Students use real data collected from the field in Borneo to test whether Guardian Frogs are the first known amphibian with sex role reversal (caring males and competitive females).

  1. Presentation

  2. Student Worksheet

  3. Teacher Worksheet

4. Procedure

Part 1: 60 min, Part 2: 45 min, Part 3: 45 min

There is a single worksheet packet for Parts 1 & 2. Part 1 (Data collection) will likely take a full class period. Part 2 (Analysis) can be done in another class period or independently. Students should be able to complete Part 3 in 1 class period.

Warm-Up
05101520253035404550556010 min.
1

In animals, which sex is strong, caring, competitive, or protective?

A quick activity to get students thinking about the behavioral roles that males and females play across the animal kingdom.

Students will pick one of the supplied adjectives and categorize it to one of the sexes, and provide an example of a nonhuman animal that demonstrates this adjective. The idea is to have students think about their preconceptions about sex roles. We define it here for you, but won't introduce this term until part 2. A reason for this is that the utility of the term sex roles itself is being argued about in the scientific literature.
We want students to understand that there is a general pattern of how male and female animals tend to behave, but these are not actually prescriptive roles and there is a great deal of variation within and across species.

Vocab
  • sex roles: in nonhuman animals, behaviors and attributes that typically apply to one sex or the other
  • conventional sex roles: "caring females" and "competitive males"; the pattern in most animal species wherein females contribute more to ensure the survival of offspring, while males spend more effort toward attracting and competing for mates
  • sex-role-reversal: "caring males" and "competitive females"; this is a less common pattern among animals, in which males contribute more to parental care and females compete for access to mates

Make sure to emphasize that we're not talking about humans. Later, we will also draw the distinctions between gender and sex.

2

Share out

Let students share a bit of their knowledge and perhaps begin to disagree about classifications. We want them to start thinking about how complex and variable animal mating systems are.

Intro
05101520253035404550556010 min.
3

Across animals, is one sex more involved in caring for young, while the other is focused on competing for mates?

Set up our driving question and define the language we'll be using.

Vocab
  • sex: a category (male or female) determined by which gametes an individual produces (i.e. eggs for females and sperm for males)
  • gender: a complex social category based on how an individual identifies (e.g. male, female, nonbinary)

We always use sex, rather than gender to refer to nonhuman animals, since we can't ask animals how they identify. However, there is some very interesting ongoing research looking into animal genders.

4

"Role With It" Rules

A card-based simulated data collection activity involving "song" and dance

Students each get at least 1 card from the ♧'"Role With It" Cards'. This card is their "specimen" (male or female of a species), which they will introduce to the class as they collect its data. Each card has provided sexual signal values (from 0 to 3) for visual and acoustic (sound) traits. Zero means a specimen doesn't have that type of signal; 3 means that sex of that species has a very exaggerated trait. Students will build a dataset of relative sexual signal elaboration (e.g. how fancy the feathers and songs are) and parental investment for each sex across 12 different species.
The presentation guides the game. When a specimen's common name is called, the student with that card must stand up and introduce it. They will repeat their name 3-5 times at a volume corresponding to the Acoustic Signal Score on their card. Simultaneously, they will do a dance that is at the level of the Visual Display Score on their card.
After the introduction, the student will read for their classmates the Parental Investment information on the card. Everyone then records display scores and parental contributions, which they will use for futher analysis. Then the next card is introduced.

Vocab
  • signals: traits evolved to communicate something to a receiver (e.g. "danger," "I'm here," or "mine")
  • sexual signals: traits evolved specifically to communicate in the context of mate attraction, competition, or both
  • visual signals: traits evolved to communicate by sight (e.g. feather shapes and colors; antlers; or bright patches of skin)
  • acoustic signals: traits evolved to communicate through sound (e.g. bird and whale song; cricket chirps; or frog calls)

We've included 30sec timers in the presentation to keep things moving along, as students will find much of the goings on quite entertaining.
It's a good idea to demonstrate the "song" and dances, and set rules for what a "3" means in terms of movement and volume. One of us demoed a floss and while one student "died of cringe," another confided that it made her day. Have fun! :)

5

Pass out cards and let students trade

Each student should have at least 1 card. In small classes, students may have several. In a large class (>24), you will need to have more than 1 deck to allow each student to have a card. If you have multiple students introducing a specimen, you should call on one to read the Parental Contributions section.

Once they have the data, Part 2 can be done independently for homework or during another class period.

"Role with It" Data Collection
05101520253035404550556035 min.
6

Students will make 24 small performances as they introduce their species, followed by data collection.

This will be somewhat of a chaotic lesson, but students should definitely find it engaging. They will enjoy performing and seeing their classmates perform.

Everything downstream requires that all students collect the data they are introducing. Students will likely need periodic reminders to focus and take down the data to move on. We've added the timers as a reminder.

Vocab
  • dewlap: the extendable flap under some lizards' necks (e.g. in green anoles), which is used for competition and mate attraction
  • wattle: a fleshy flap of skin on some birds (such as turkeys and jacanas) that likely plays a role in mate choice

We include short videos after some of the species have been introduced to show students their actual display behaviors. Make sure you have the sound on! 🔊

Wrapping Up Data Collection
0510152025303540455055605 min.
7

Collect work to finish later in class or assign homework

If you finished, have students make sure they've totaled all their scores in Table 1.

If you don't quite get through the P1 Presentation, simply collect student papers (you can have them place cards on top of their worksheet packet and stack them). They should have recorded which cards they had on Q1-5, as well, so it's straightforward to pick up where you left off on data collection at the last species in Table 1 on the worksheet.

Once they have the data, the rest can be done independently for homework or during another class period, depending how you'd like to support your students.

(Re)organizing the Data
05101520253035404515 min.
1

Transferring raw data to a summary table

What do the data say about our question: Across animals, is one sex more involved in caring for young, while the other is focused on competing for mates?

Students will copy their Total Sexual Signaling Scores and Total Parental Investment Scores from Table 1 to Table 2. They will then right the correct symbol (<, >, or =), according to how male and female scores compare to each other.

There is no presentation for Part 2. The worksheet should guide students in analysis of their data.

You may want to have students work in pairs on this part in order to help them troubleshoot and spot each other's mistakes.

2

Validate the data

In order to make your life easier, and introduce students to an important computer science concept, we've implemented a checksum for Table 2.

After reorganizing their raw data in Table 2, students should add up each category of scores to get totals. Students will know if their data is valid (correct) if the column totals equal the expected checksums. If not, they should work with a classmate to find the error before coming to you to troubleshoot.

Vocab
  • checksum: in computer science, this is a value used to determine if data are valid. If a calculation does not result in the expected checksum, it is a sign that something went wrong. (e.g. checksums are used to determine if you've put in a valid credit card number)
Analysis and Reflection
05101520253035404530 min.
3

Independent work

The worksheet guides students through a basic summary analysis and reflection on the data.

What students should independently learn from this analysis:

  • there is a lot of variation in nature
  • when one sex contributes significantly more parental investment, it frees the other sex to evolve adaptations to compete for mates
  • while the majority of species have "caring females" and "competitive males" (conventional sex roles), there are many cases where both parents contribute equivalently, and some species show sex role reversal
Where in the world is Borneo?
05101520253035404510 min.
1

Immersion in a tropical forest

As students are streaming into the class, have Slide 2 playing the ▶'Borneo Atmospheric Video' with sound on 🔊

This looping video alternates between showing a planetary view of where we're going in this trip to sights and sounds from the field.

The graphic scrolling along the bottom of the video is a sound spectrogram. It shows frequency along the vertical Y axis and time scrolling along the X axis.

2

Geopolitical boundaries

A few slides check in with your students' geographical knowledge and show the 3 countries that claim the island of Borneo: Malaysia, Indonesia, and the tiny sultanate of Brunei. The rest of the lesson will focus on the research Dr. Johana Goyes Vallejos conducted in Brunei.

Quick Review
0510152025303540455 min.
3

What did we learn yesterday?

Slides prompt a quick review of the general pattern that students found in their data in Part 2.

Here we introduce the term sex roles and conventional sex roles in particular to describe the finding that in 75% of the species we looked at in Parts 1-2, males were "competitive" and females were "caring." We then highlight the exceptional species (pipefish, jacanas, and guardian frogs).

Vocab
  • sex roles: in nonhuman animals, behaviors and attributes that typically apply to one sex or the other
  • conventional sex roles: "caring females" and "competitive males"; the pattern in most animal species wherein females contribute more to ensure the survival of offspring, while males spend more effort toward attracting and competing for mates
  • sex-role-reversal: "caring males" and "competitive females"; this is a less common pattern among animals, in which males contribute more to parental care and females compete for access to mates
Setting Up for Student Analysis
05101520253035404510 min.
4

Watch Video

Students learn about Johana and the motivations for her research with ▶'Calling behavior of male and female Guardian Frogs'

This video sets up the background information and sets up the driving question for Part 3: "Is the Smooth Guardian Frog fo Borneo the first sex-role-reversed frog species known to science?"

5

Introduce the criteria for sex role reversal

Students will determine if the Guardian Frogs meet a set of 3 criteria to be considered the first sex role reversed frog species. Two are provided in the background information (Males are known to provide parental care by guarding eggs and transporting tadpoles to water; females are also known to call, which is normally a trait only found in males). Students will spend the rest of the class time testing out the third criteria: Do females signal more often than males?

Independent Work
05101520253035404520 min.
6

Students complete the worksheet packet on their own

Students add missing labels to a graph of male and female call rates.

In order to complete the primary task, students will have to compare the units of the X and Y axis on a scatter plot to the values in the table in order to figure out how to label the figure. They will also have to compare the relative values of males and females in order to label the legend key. Once they have figured out the main task, students are asked to interpret whether the data meet criterion 3: that females call more than males (they do). Finally, students are asked to write a persuasive paragraph synthesizing what they have learned to explain why guardian frogs deserve more research funding and what will be lost if they are allowed to go extinct.

When you finish teaching this lesson, we would really love to hear what you thought! Please fill out a simple survey in the Feedback section below.

5. Background

Connection to Research

Instead of telling students that a pattern exists, we have them discover it for themselves. That is, rather than telling them, "In animal species with conventional sex roles, males invest more in attracting and competing for mates, while females invest more in parental care," we have students collect data in a fun, interactive format and draw this conclusion from the data. They are then more invested in understanding cases of sex role reversed species that are the exceptions to this rule, and have "caring males" and "competitive females."

In part 2, students follow in the footsteps of an early career Latina scientist, Dr. Johana Goyes Vallejos, testing the same hypotheses she did during her PhD research. Students analyze a real graph and table she collected on the island of Borneo to determine if the Smooth Guardian Frog (Limnonectes palavanensis) is the first sex-role-reversed frog species known to science. (It is!)

Research Background

Watch ▶'Do Female Frogs Call?' to get a nice summary of Dr. Johana Goyes Vallejos’ scientific journey.

While pursuing a PhD in ecology and evolutionary biology at the University of Connecticut, Johana heard about a poorly studied frog called the Smooth Guardian Frog (Limnonectes palavanensis), found only on the island of Borneo in Southeast Asia. In a couple of scientific papers in the 1980s, a researcher had described a highly unusual behavior--the males guard eggs hidden under the leaf litter (thus, the common name of the species). When the eggs hatch, the male then carries the tadpoles (on his back!) to small pools of water where they can develop into adult frogs.
Johana, who was deeply interested in parental care behavior decided to fly to the tiny sultanate of Brunei (ruled by a sultan, rather than a king) and learn about this frog herself. She established a study population, tracking individuals and recording audio of frog calls, often on her own at night, wading through thick mud and fording streams. During her observations, she noticed a tiny peep. It sounded very different from the male calls she had been used to hearing. After careful observation, she confirmed that indeed, these were female calls, which had never been described before by anyone. (See ▶'Calling behavior of male and female Guardian Frogs')

Out of 8,000+ known frog species, Johana had found one of only a couple that are known to have calling females. Previously, scientists had thought females might be physiologically incapable of producing calls. In contrast, when she started focusing on female calling behavior, she found that they were actually calling more than males! Students will recapitulate this finding by analyzing a figure showing male and female calling rates.

As Borneo is now being heavily deforested, populations of Guardian Frogs are declining. In the final assignment, students are asked to synthesize everything they’ve learned to write a persuasive paragraph about why these exceptional frogs matter and what we stand to lose if they are lost.

Researcher Bio

Dr. Johana Goyes Vallejos is a Preparing Future Faculty for Faculty Diversity Postdoctoral Fellow in the Division of Biological Sciences at the University of Missouri, Columbia. She graduated with a B.Sc. in Biology with a minor in Genetics from the Universidad del Valle in Cali, Colombia. She received her Ph.D. in Ecology and Evolutionary Biology from the
University of Connecticut, studying mating systems, parental care behavior, and acoustic
communication in frogs. Her research has taken her to many tropical forests across the
world, including Panama, Costa Rica, Guyana, the Philippines, Malaysia and Brunei
Darussalam.

At the University of Missouri, Dr. Goyes Vallejos continues her research on
mating behavior and parental care strategies using frogs with elaborate parental behaviors
as study systems. In addition, since 2018, she serves as a research mentor for an NSF-
funded Research Experience for Undergraduates program in Costa Rica, providing
hands-on training in animal behavior and field research to undergraduate students
belonging to minoritized groups in STEM. She is passionate about sharing her research findings with scientific and non-scientific audiences through various media outlets. When not chasing frogs in remote forests around the world Dr. Goyes Vallejos enjoys salsa dancing, storytelling and crocheting amigurumis.

Learn more about Johana at her website

Further Reading and Listening

Related Scientific Paper

Screenshot of scientific journal article
Link to study

6. Learning Standards

This Galactic Polymath Learning Chart illustrates the areas of knowledge covered. This lesson targets Science, but it helps teach national learning standards in 4 subjects:

Notes on Standards

*Standards are broken down into Target and Connected categories. Target standards are directly reinforced or taught; connected standards are not fully addressed in the lesson, but connected enough to provide a foundation for teachers to build upon.

Learning Standards

Note: Click on any standardfor details on how the lesson aligns to it.

Target Standard(s)

Science - NGSS

Dimension: Disciplinary Core ideas

How does the lesson align to this standard?
  • Students will discuss why animals reproduce, and what types of behavior animals engage in to increase the odds of reproduction, and, after an overview of typical behavior, we will discuss examples of exceptions to the rule, such as sex role reversal.

Dimension: Science & Engineering Practices

How does the lesson align to this standard?
  • Students will integrate data visualization of real biological data and scientific text, using scientific ideas and principles to investigate an real example of exceptions to the rule in nature, with evidence for sex role reversal in Guardian Frogs.

Dimension: Cross-Cutting Concepts

How does the lesson align to this standard?
  • Students will practice looking at real data that is variable, and become familiar with looking at mean and variability, through experience plotting boxplots and follow-up interpretation of data and discussion.

Connected Standard(s)

Dimension: Measurement, Data, Probability & Statistics

How does the lesson align to this standard?
  • Students will graph data, and become familiar with plotting the center and spread with boxplots, using real data on Guardian Frog behavior.
How does the lesson align to this standard?
  • Students will practice graphing real biological data that is variable, and in so doing, will gain experience displaying numerical data in plots on a number scale, such as box plots.

Dimension: Language, Speaking & Listening

How does the lesson align to this standard?
  • Students will be presented with data visualizations, including graphs, along with oral and textual scientific information, and will be asked to interpret this information to understand the relationship between animal behavior and sexual selection.

Dimension: Reading

How does the lesson align to this standard?
  • Students will integrate their findings from data visualization in graphs along with a piece of scientific text on Exceptions in Nature.
How does the lesson align to this standard?
  • Students will read a secondary source on Exceptions in Biology, from which we will ask them to draw on specific citations in their discussion of why exceptions in biology matter, and how they relate both specifically to our discussion of Guardian Frogs, and more broadly to the field of biology and beyond.

Dimension: Science & Engineering Practices

How does the lesson align to this standard?
  • Students will plot real biological data in boxplots and interpret their data visualization to provide evidence to test their hypotheses about sex role reversal.
How does the lesson align to this standard?
  • Students will practice looking at real data that is variable, and become familiar with looking at mean and variability, through experience plotting boxplots and follow-up interpretation of data and discussion.
How does the lesson align to this standard?
  • Students will practice looking at real data that is variable, and become familiar with looking at mean and variability, through experience plotting boxplots and follow-up interpretation of data and discussion.
How does the lesson align to this standard?
  • Students will read a scientific text on Exceptions in Biology, which they will summarize and interact with to understand why exceptions to the rule matter in biological systems.

Dimension: Cross-Cutting Concepts

How does the lesson align to this standard?
  • Students will plot real biological data in boxplots and interpret their data visualization to provide evidence to test their hypotheses about sex role reversal.

Dimension: Disciplinary Core ideas

How does the lesson align to this standard?
  • As an extension, students may investigate the relationship between biodiversity and geography, learning the definition of biodiversity and how habitat destruction and fragmentation can impact biodiversity, as is the case with the Guardian Frogs of Borneo.

Dimension: Civics, Economics, Geography & History

How does the lesson align to this standard?
  • Students will investigate a map of Borneo that overlays environmental characteristics, from which they can make interpretations about the relationship between geography and biodiversity, and how these might be impacted by habitat loss.

7. Feedback

8. Credits

Lead Author and Creative Director:

Matt Wilkins, PhD
If you liked this lesson, please help spread the word! Feel free to tag me (@mattwilkinsbio) and GP (@galacticPM) on Twitter with your experiences and student questions!

And please sign up for our mailing list to hear what’s coming up next!

Sponsor and Producer

Johana Goyes Vallejos, PhD

"Role with It" Animal Diversity Cards Illustrator

Anna Wilkins

Education Consultant

(Initial lesson development)

Tracy Burkhard, PhD
Postdoctoral Fellow
Centre d’Ecologie Fonctionnelle et Evolutive
Montpellier, France
Rachel Neurath, PhD
Soil Microbial Ecologist | STEM Educator | Writer
New England, USA

Major Release Beta

0.1.0 Initial lesson build.

Apr 02, 2022

Major Release 1

1.0.0 Worldwide release!

Apr 22, 2022

1.0.1 Tweaks to acknowledgments & procedure

Apr 22, 2022